Characterization of a Streptomyces-lividans-type site-specific DNA modification system in the avermectin-producer Streptomyces avermitilis permits investigation of two novel giant linear plasmids, pSA1 and pSA2
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چکیده
منابع مشابه
Construction of a single component producer from the wild type avermectin producer Streptomyces avermitilis.
Most microbial secondary metabolites are producec as complexes of closely related compoundsby theii producing organisms. The avermectins1*, milbemycins2 and nemadectins3~5) are typical examples produced ii such a manner. The milbemyciif complex is known t( consist of more than twenty components6 ~ 8), however only a few are commercial products. A rationale for thii phenomenon may be related to ...
متن کاملthe investigation of the relationship between type a and type b personalities and quality of translation
چکیده ندارد.
Cloning and analysis of a DNA fragment stimulating avermectin production in various Streptomyces avermitilis strains.
To isolate a gene for stimulating avermectin production, a genomic library of Streptomyces avermitilis ATCC 31267 was constructed in Streptomyces lividans TK21 as the host strain. An 8.0-kb DNA fragment that significantly stimulated actinorhodin and undecylprodigiosin production was isolated. When wild-type S. avermitilis was transformed with the cloned fragment, avermectin production increased...
متن کاملSAV742, a Novel AraC-Family Regulator from Streptomyces avermitilis, Controls Avermectin Biosynthesis, Cell Growth and Development
Avermectins are useful anthelmintic antibiotics produced by Streptomyces avermitilis. We demonstrated that a novel AraC-family transcriptional regulator in this species, SAV742, is a global regulator that negatively controls avermectin biosynthesis and cell growth, but positively controls morphological differentiation. Deletion of its gene, sav_742, increased avermectin production and dry cell ...
متن کاملIsolation, Characterization and Selection of Avermectin-Producing Streptomyces avermitilis Strains From Soil Samples
BACKGROUND Streptomyces avermitilis, belonging to Actinomycetes, is specialized for production of avermectin, used as an anthelmintic and insecticidal agent. It is mostly found in soil and its isolation is very crucial for medically important avermectin production. OBJECTIVES In the present study, 10 bacterial isolates lacking antimicrobial activities were isolated from the soil samples colle...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1994
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-140-6-1367